Table 1.

Characterization of ATP uptake by platelets membrane fraction

Compounds (μmol/L)Uptake, % of control
InhibitorsControl100.0 ± 3.0
Evans Blue1057.9 ± 5.7
DIDS1029.4 ± 10.9
Bafilomycin A10.0535.8 ± 3.1
Cl dependencyControl4000100.0 ± 19.7
056.6 ± 22.2
cis‐inhibitionControl100.0 ± 5.2
ATP500023.3 ± 0.3
ADP50003.6 ± 2.3
AMP500066.4 ± 2.5
AMP‐PNP500046.0 ± 5.0
KH2PO4500045.3 ± 3.3
  • The effect of inhibitors, Cl, and nucleotides on ATP uptake by platelets membrane fraction after 5 min. Control activity (100%) corresponds to 6.7 ± 0.3 nmol/mg protein. For Cl dependence, an equivalent concentration of the potassium acetate in the reaction mixture was replaced with the indicated concentration of KCl. Data are mean ± SD, n = 3.