Table 3. Fatty acid composition of phospholipids in Huh7 and HepG2 cells cultured with 2% HS or 10% FBS
HSFBS
Huh7HepG2Huh7HepG2
Saturated fatty acids
14:00.90 ± 0.103.76 ± 0.69**,0.83 ± 0.236.38 ± 1.74**,
16:031.2 ± 1.5035.8 ± 2.1*30.5 ± 1.5033.4 ± 1.6*
18:013.1 ± 1.305.1 ± 0.4**11.5 ± 1.205.7 ± 0.9**
Monounsaturated fatty acids
16:1n‐78.50 ± 1.869.75 ± 1.629.20 ± 2.0313.85 ± 4.58*
18:1n‐920.9 ± 1.215.9 ± 1.4**,29.2 ± 2.419.0 ± 1.3**,
18:1n‐77.0 ± 1.514.9 ± 3.4**5.4 ± 0.312.5 ± 3.3**
20:1n‐90.29 ± 0.140.57 ± 0.07*0.29 ± 0.140.73 ± 0.06**
Polyunsaturated fatty acids
18:2n‐65.16 ± 0.165.58 ± 0.560.49 ± 0.050.69 ± 0.12
18:3n‐60.47 ± 0.070.26 ± 0.02**,0.19 ± 0.030.18 ± 0.05
18:3n‐30.07 ± 0.0200.1 ±  <0.010.16 ± 0.010.09 ± 0.04**
20:4n‐62.79 ± 0.611.51 ± 0.26**1.70 ± 0.190.97 ± 0.04*
20:5n‐30.38 ± 0.050.48 ± 0.140.61 ± 0.040.31 ± 0.12**,
22:5n‐31.22 ± 0.290.57 ± 0.05**1.49 ± 0.250.92 ± 0.25*
22:6n‐31.21 ± 0.481.77 ± 0.36*,1.04 ± 0.060.84 ± 0.12
  • Cells were cultured in either 2% human serum (HS) or 10% fetal bovine serum (FBS)‐containing media for 7 days. Data are mean ± SD, = 5. Two‐way ANOVA with Bonferroni's post hoc tests. *< 0.05, **< 0.001 versus Huh7 cells in the same serum condition; matching symbols denote significant differences between means for Huh7 () and HepG2 cells (), < 0.05.