Table 1. mRNA expression of lipid metabolism genes in Huh7 and HepG2 cells cultured with 2% HS or 10% FBS
HSFBS
Huh7HepG2Huh7HepG2
FA transport and synthesis
ACACA 0.67 ± 0.051.27 ± 0.12**0.74 ± 0.081.32 ± 0.10**
ACACB 0.96 ± 0.161.60 ± 0.50*,0.86 ± 0.051.00 ± 0.11
CIDEC 1.40 ± 0.300.32 ± 0.05**0.77 ± 0.190.23 ±  <0.01*
DGAT2 1.52 ± 0.341.04 ± 0.061.47 ± 0.430.66 ± 0.15*
FASN 0.60 ± 0.061.01 ± 0.13*, 0.66 ± 0.081.35 ± 0.23**,
FABP1 1.44 ± 0.310.71 ± 0.05*1.37 ± 0.120.68 ± 0.08*
SCD1 0.98 ± 0.211.30 ± 0.091.03 ± 0.221.40 ± 0.28
FA elongation and desaturation
ELOVL2 0.77 ± 0.061.75 ±  <0.01**,0.85 ± 0.101.15 ± 0.15*,
ELOVL5 0.93 ± 0.141.07 ± 0.021.03 ± 0.111.22 ± 0.05
ELOVL6 1.27 ± 0.170.80 ± 0.12*1.56 ± 0.190.85 ± 0.08**
FADS1 1.10 ± 0.220.60 ± 0.07*1.22 ± 0.270.59 ± 0.10*
FADS2 1.45 ± 0.180.51 ± 0.05**1.58 ± 0.240.51 ± 0.09**
Lipolysis, secretion, and oxidation
APOB 0.65 ± 0.071.70 ± 0.26**,0.60 ± 0.120.93 ± 0.05*,
CES1 1.45 ± 0.140.01 ±  <0.01**2.25 ± 0.190.01 ±  <0.01**
CIDEB 1.14 ± 0.210.21 ± 0.02**0.98 ± 0.260.20 ± 0.08*
CPT1A 1.22 ± 0.250.59 ± 0.07*1.37 ± 0.240.66 ± 0.05**
LIPC 1.34 ± 0.280.32 ± 0.06**1.03 ± 0.350.18 ± 0.04*
MTTP 1.63 ± 0.400.74 ± 0.01*1.47 ± 0.100.39 ± 0.04**
PNPLA2 0.52 ± 0.091.80 ± 0.28**,0.54 ± 0.081.12 ± 0.15**,
PNPLA3 1.26 ± 0.100.92 ± 0.12**1.17 ± 0.100.54 ± 0.04*
TM6SF2 1.57 ± 0.070.55 ± 0.10**1.19 ± 0.170.41 ± 0.05*
Hepatic nuclear receptors
HNF4A 1.25 ± 0.211.11 ± 0.121.22 ± 0.210.83 ± 0.04*
NR1H3 1.33 ± 0.080.99 ± 0.22*1.21 ± 0.230.70 ± 0.22**
NR1H4 1.29 ± 0.220.55 ± 0.071.30 ± 0.340.19 ± 0.08*
Transcription factors
MLXIPL 0.19 ± 0.062.68 ± 0.97**,0.19 ± 0.031.11 ± 0.14*,
PPARA 1.19 ± 0.140.76 ± 0.11*1.03 ± 0.190.64 ± 0.10*
PPARG 1.08 ± 0.260.48 ± 0.22*1.03 ± 0.230.28 ± 0.06*
PPARGC1A 0.02 ±  <0.012.78 ± 0.27**0.01 ±  <0.012.46 ± 0.54**
SREBF1 0.92 ± 0.050.62 ± 0.04*0.83 ± 0.150.75 ± 0.04
  • Cells were cultured in either 2% human serum (HS) or 10% fetal bovine serum (FBS)‐containing media for seven days. mRNA expression is presented relative to a calibrator sample, all genes were corrected to three reference genes (B2M, HPRT1, and YWHAZ). Data are mean ± SD, = 4. Two‐way ANOVA with Bonferroni's post hoc tests. *< 0.05, **< 0.001 versus Huh7 cells in the same serum condition; matching symbols denote significant differences between means for Huh7 () and HepG2 cells (), < 0.05.