Table 1. Effect of pharmacological inhibitors on sensitivity (pEC50) and maximal relaxing responses (Emax) to ACh in mesenteric resistance arteries from young and aged mice on a normal chow (NC) and a high fat sucrose (HFS) diet
TreatmentParameterNC youngNC agedHFS youngHFS aged
No inhibitorspEC50 (−log M)6.60 ± 0.056.05 ± 0.09*6.04 ± 0.07*4.66 ± 0.14*,#
Emax (%)91 ± 279 ± 875 ± 4*37 ± 9*,#
IpEC50 (‐ log M)6.19 ± 0.06**5.90 ± 0.136.13 ± 0.084.59 ± 0.21*,#
Emax (%)86 ± 474 ± 1278 ± 532 ± 10*,#
T+U+IpEC50 (−log M)5.79 ± 0.05**5.60 ± 0.07**5.54 ± 0.07**4.67 ± 0.23*,#
Emax (%)67 ± 3**66 ± 6**60 ± 4**37 ± 9*,#
L‐N+IpEC50 (−log M)5.49 ± 0.11**5.36 ± 0.18**4.96 ± 0.09**4.27 ± 0.90*,#
Emax (%)55 ± 6**54 ± 14**42 ± 7**13 ± 11*,**,#
L‐N+I+ebselenpEC50 (−log M)5.74 ± 0.13**5.97 ± 0.196.13 ± 0.065.61 ± 0.15**
Emax (%)72 ± 7**67 ± 1673 ± 463 ± 9*
  • The sensitivity (pEC50) of ACh is calculated with GraphPad Prism6 software as described in the methods and denotes the negative logarithmic concentration of ACh that induces 50% relaxation compared to the maximal relaxation (Emax). Abbreviations used: I (indomethacin), L‐N (L‐NAME), T (TRAM‐34), and U (UCL1684). See text for inhibitor concentrations used. Values are shown as means ± SEM. *< 0.05 versus NC Young, #< 0.05 versus HFS Young, **< 0.05 versus no inhibitors.