Table 2. Phases of the cell cycle in pressurized HKC‐8 and NRK‐52E cells
Cell cycle phaseSerum‐free HKC‐8 cellsComplete HKC‐8 cells
0 mmHg (%)60 mmHg (%)90 mmHg (%)0 mmHg (%)60 mmHg (%)90 mmHg (%)
24 h
G0/G149.9 ± 0.344.5 ± 1.5a48.1 ± 1.237.8 ± 1.735.6 ± 1.034.9 ± 1.7
S34.5 ± 0.138.6 ± 1.4a36.0 ± 1.043.1 ± 1.045.0 ± 0.847.0 ± 1.1
G2/M13.0 ± 0.714.1 ± 0.514.0 ± 0.316.9 ± 0.917.2 ± 0.915.5 ± 0.7
48 h
G0/G154.8 ± 0.649.7 ± 0.5a50.0 ± 1.6a35.8 ± 1.035.1 ± 0.832.3 ± 0.1
S29.7 ± 1.134.6 ± 0.5a34.6 ± 0.5a44.9 ± 2.145.7 ± 0.548.4 ± 0.8
G2/M12.6 ± 1.012.8 ± 0.713.0 ± 0.716.1 ± 1.216.4 ± 0.216.7 ± 1.1
Cell cycle phaseSerum‐free NRK‐52E cellsComplete NRK‐52E cells
0 mmHg (%)60 mmHg (%)90 mmHg (%)0 mmHg (%)60 mmHg (%)90 mmHg (%)
24 h
G0/G152.7 ± 3.446.6 ± 0.744.2 ± 2.3a38.7 ± 2.533.7 ± 0.430.8 ± 4.1
S37.1 ± 1.946.6 ± 0.7a46.0 ± 1.7a52.3 ± 2.257.0 ± 1.158.0 ± 2.4
G2/M10.3 ± 1.58.2 ± 0.18.0 ± 0.58.8 ± 0.811.0 ± 0.911.5 ± 0.2
  • The percentage of cells in each phase of the cell cycle is shown in pressurized cells cultured in either serum‐free medium (DMEM only) or in complete medium (DMEM + 10% FBS) at each time point. Data represents mean ± SEM.

  • a P < 0.05 compared to zero pressure. N = 3, with triplicate replications in each experiments.