Table 3. Phases of the cell cycle in stretched HKC‐8 and NRK‐52E cells
Cell cycle phaseSerum‐free HKC‐8 cellsComplete HKC‐8 cells
Unstretched (%)Stretched (%)Unstretched (%)Stretched (%)
24 h
G0/G150.2 ± 2.848.8 ± 1.243.1 ± 1.739.9 ± 0.5
S29.7 ± 0.532.6 ± 0.940.2 ± 0.440.7 ± 0.5
G2/M20.1 ± 0.918.0 ± 1.018.6 ± 1.319.0 ± 0.8
48 h
G0/G153.3 ± 2.854.8 ± 1.150.2 ± 2.045.9 ± 1.0a
S27.6 ± 0.528.4 ± 0.330.2 ± 1.133.7 ± 0.4a
G2/M18.8 ± 1.118.0 ± 0.419.8 ± 0.721.4 ± 1.7
Cell cycle phaseSerum‐free NRK‐52E cellsComplete NRK‐52E cells
Unstretched (%)Stretched (%)Unstretched (%)Stretched (%)
24 h
G0/G167.2 ± 2.853.9 ± 3.252.5 ± 2.845.4 ± 2.2a
S21.4 ± 2.126.0 ± 1.933.6 ± 2.436.3 ± 1.9a
G2/M11.5 ± 1.314.1 ± 1.516.1 ± 1.216.1 ± 1.1
  • The percentage of cells in each phase of the cell cycle is shown in pressurized cells cultured in either serum‐free medium (DMEM only) or in complete medium (DMEM + 10% FBS) at each time point. Data represents mean ± SEM.

  • a P < 0.05 compared to unstretched cells. N = 3, with triplicate replications in each experiments.