Table 2. Body composition and glucose metabolism of three age groups of mice after 9 weeks of high fat diet feeding. At the onset of the dietary intervention mice were either at the age of 8 weeks (adolescent), 16 weeks (young adult), or 52 weeks (middle‐aged)
AdolescentYoung adultMiddle‐agedTwo‐way ANOVA
ControlHigh fatControlHigh fatControlHigh fatP(Age)P(Diet)P(AgeDiet)
Body mass initial21.4 ± 0.921.2 ± 0.926.6 ± 0.8126.1 ± 0.9130.2 ± 0.71,230.2 ± 1.11,2<0.001n.s.n.s.
Body mass final24.7 ± 0.729.5 ± 1.0327.8 ± 0.7136.0 ± 2.41,330.7 ± 0.91,243.7 ± 2.01,2,3<0.001<0.001<0.001
Body mass gain3.2 ± 0.78.3 ± 1.131.2 ± 0.79.9 ± 2.330.4 ± 0.6113.5 ± 2.41,3n.s.<0.001<0.001
Fat mass initial1.7 ± 0.21.8 ± 0.22.9 ± 0.512.7 ± 0.113.1 ± 0 513.4 ± 0.41,2<0.001n.s.n.s.
Fat mass final2.1 ± 0.36.6 ± 0.833.1 ± 0.411.8 ± 1.71,33.5 ± 0.617.3 ± 1.21,2,3<0.001<0.001<0.001
Fat mass gain0.4 ± 0.24.9 ± 0.830.2 ± 0.39.1 ± 1.81,30.4 ± 0.813.9 ± 1.21,2,3<0.001<0.001<0.001
Lean mass initial16.1 ± 0.915.7 ± 0.819.6 ± 0.4119.4 ± 0.6122.7 ± 0.81,222.3 ± 0.71,2<0.001n.s.n.s.
Lean mass final18.3 ± 0.819.5 ± 0.620.2 ± 0.6119.8 ± 0.621.7 ± 0.51,221.2 ± 0.71,2<0.001n.s.0.003
Lean mass gain2.2 ± 0.33.8 ± 0.60.6 ± 0.610.4 ± 0.31−1.0 ± 1.11,2−1.1 ± 1.41,2<0.001n.s.0.02
Adjusted fat mass42.7 ± 0.411.6 ± 1.033.0 ± 0.312.1 ± 0.532.9 ± 2.912.0 ± 1.53n.s.<0.001n.s.
Adjusted lean mass420 ± 0.520.4 ± 0.520.2 ± 0.419.8 ± 0.520.0 ± 0.720.3 ± 0.7n.s.n.s.n.s.
Fasting Glc Final7.7 ± 1.710.4 ± 0.939.4 ± 0.5110.1 ± 1.47.6 ± 0.429.3 ± 1.130.03<0.001n.s.
Fasting Insulin0.6 ± 0.31.3 ± 0.630.6 ± 0.31.5 ± 0.531.0 ± 0.44.2 ± 1.11,2,3<0.001<0.001<0.001
Total AUC5177 ± 39275 ± 323229 ± 33321 ± 633227 ± 35317 ± 2330.007<0.001n.s.
Liver mass1.3 ± 0.11.2 ± 0.21.2 ± 0.11.5 ± 0.31.6 ± 0.11,21.7 ± 0.21<0.001n.s.n.s.
Adjusted liver mass41.5 ± 0.11.2 ± 0.21.3 ± 0.11.4 ± 0.31.6 ± 0.11.4 ± 0.2n.s.n.s.n.s.
  • n = 6 per diet and age group. Data are represented as means ± SD. Two‐Way ANOVA statistics for P(Age) – age effect, P(Diet) – diet effect, and P(AgeDiet) – interaction. n.s., not significant (P ≥ 0.05).

  • Results of post hoc testing are indicated by 1Significantly different to Adolescent within feeding group; 2Significantly different to Young Adult within feeding group; 3Significantly different to age‐matched control group.

  • 4Adjustment for final body mass as covariate by ANCOVA.

  • 5AUC – area‐under‐the‐curve of ipGTT in (min × g × L−1). Body mass, fat mass and lean mass, initial and final, body mass gain, fat mass gain, lean mass gain, adjusted fat mass, adjusted lean mass, liver mass, and adjusted liver mass – (g); fasting glucose – (mmol/L); fasting insulin – (ng × mL−1).