Table 3. Oxygen consumption in isolated liver mitochondria of adolescent, young adult, and middle‐aged mice
Oxygen consumption4AdolescentYoung adultMiddle‐agedTwo‐way ANOVA
ControlHigh fatControlHigh fatControlHigh fatP(Age)P(Diet)P(AgeDiet)
Succ−ADP13 ± 615 ± 727 ± 821 ± 550 ± 161,258 ± 191,2<0.001n.s.n.s.
+ADP47 ± 1942 ± 1576 ± 2857 ± 10127 ± 231,2155 ± 461,2<0.001n.s.n.s.
RCR3.8 ± 0.33.0 ± 0.72.7 ± 0.512.7 ± 0.32.7 ± 0.612.7 ± 0.20.002n.s.n.s.
PC/Mal−ADP12 ± 310 ± 216 ± 414 ± 4122 ± 51,220 ± 41,2<0.001n.s.n.s.
+ADP69 ± 1547 ± 7383 ± 1182 ± 25195 ± 23175 ± 91,30.0020.02n.s.
RCR6.0 ± 1.04.9 ± 1.05.4 ± 1.55.8 ± 1.44.3 ± 0.513.8 ± 0.720.002n.s.n.s.
  • RCR, respiratory control ratio.

  • Results of post hoc testing are indicated by 1Significantly different to Adolescent within feeding group;

  • 2ignificantly different to Young Adult within feeding group;

  • 3Significantly different to age‐matched control group.

  • 4nmol O2 × mg−1 × min−1, Succ, succinate/rotenone, PC/Mal, palmitoylcarnitine/Malate.

    Data are represented as means ± SD; n = 5–6 per group; Two‐Way ANOVA statistics for P(Age) ‐ age effect, P(Diet) ‐ diet effect, and P(AgeDiet) ‐ interaction.