Table 2. Measured surface area of skin affected by perfused sudomotor agents via intradermal microdialysis
Protocol 1Protocol 2
Methacholine (1 mmol L−1)Methacholine (10 mmol L−1)Methacholine (100 mmol L−1)Methacholine (1000 mmol L−1)Methacholine (2000 mmol L−1)Ouabain (6 mmol L−1)Atropine (58 μmol L−1)
Reviewer 1 (cm2)0.9 ± 0.41.2 ± 0.52.3 ± 0.94.1 ± 0.85.3 ± 1.22.1 ± 0.51.2 ± 0.3
Reviewer 2 (cm2)0.9 ± 0.41.2 ± 0.62.3 ± 0.74.1 ± 1.25.2 ± 1.22.1 ± 0.71.3 ± 0.3
P value0.820.470.750.930.860.930.87
Correlation (r)0.990.980.900.840.940.940.92
Reviewer mean (cm2)0.9 ± 0.41.2 ± 0.5a2.3 ± 0.8a4.1 ± 1.0a5.2 ± 1.1a2.1 ± 0.61.3 ± 0.4
  • Values presented as mean ± standard deviation. In Protocol 1, methacholine was administered in a dose‐dependent manner and the area of activated sweat glands was measured using the modified iodine technique. In Protocol 2, ouabain and atropine were administered during 30 min of exercise at a fixed rate of metabolic heat production of 400 W and the area of inhibited sweat glands was measured using the modified iodine‐paper technique. P value represents the output of two‐tailed Student's paired t tests between values for reviewer 1 and reviewer 2. Correlation (r) represents Pearson's product–moment correlations between values for reviewer 1 and reviewer 2. Reviewer mean values are an average of the values measured by reviewer 1 and reviewer 2.

  • a Significant difference versus preceding dose in Protocol 1. Note: In Protocol 1 there were several instances of activated sweat gland areas exceeding the height and/or width of the iodine paper. Therefore, activated sweat gland area may be larger than values reported.